Plasmid

Part:BBa_J100265:Design

Designed by: Owen Koucky   Group: Campbell M Lab   (2016-06-17)


pJC173b (Carlson et al. 2014)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1813
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1813
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2482
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1813
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1813
    Illegal AgeI site found at 3618
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5458
    Illegal SapI site found at 4137


Design Notes

See "Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., 2014


Source

"Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., 2014

References